Publication: Spinach consumption ameliorates the gut microbiota and dislipaemia in rats with diet-induced non-alcoholic fatty liver disease (NAFLD)
Authors
Periago Castón, Mª Jesús ; Elvira Torales, Laura Inés ; González Barrio, Rocío ; Hidalgo, Nieves ; Navarro González, Inmaculada ; Gómez Gallego, Carlos ; Masurero, D ; Soini, E ; Vrhovsek, U. ; García Alonso, Francisco Javier
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Publisher
Royal Society of Chemistry
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DOI
https://doi.org/10.1039/C8FO01630E
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info:eu-repo/semantics/article
Description
© 2018. The authors. This document is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/
This document is the Submitted version of a published work that appeared in final form in Food and Function.
Abstract
Non-alcoholic fatty liver disease (NAFLD) is defined as the accumulation of fat in liver cells, which causes
serious health consequences. Animal and human studies suggest that the gut microbiota plays a role in
the pathogenesis of NAFLD. Here, we investigated whether spinach consumption could ameliorate highfat-
diet-induced disturbances in certain intestinal bacterial groups and products derived from their
metabolism, such as short-chain fatty acids (SCFAs) and microbial phenolic catabolites. Attention is also
paid to blood lipids and glucose. In the study, a rat model of high-fat-diet-induced NAFLD was used.
There were six experimental groups: NC (normal diet), NB (normal diet + 2.5% spinach), NA (normal diet +
5% spinach), HC (high-fat diet), HB (high-fat diet + 2.5% spinach) and HA (high-fat diet + 5% spinach). The
rats consumed these diets for five weeks, and after that, they were sacrificed and plasma, urine, intestinal
content, faeces and liver samples were taken. Biochemical parameters were analyzed in plasma, phenolic
catabolites were quantified in the faeces, urine, plasma and liver by UPLC-ESI-MS/MS, and the analysis of
the microbiota and SCFAs in the intestinal content was performed by qPCR and GLC. Consumption of a
high-fat diet caused NAFLD and dislipaemia and altered the gut microbiota and the pattern of SCFAs and
phenolic gut microbial catabolites. Supplementation with spinach partially ameliorated some alterations
induced by the high-fat diet, in particular by increasing the Lactobacillus counts, reducing the fasting
glucose and total and LDL-cholesterol and preventing excess liver cholesterol accumulation, thereby
improving the values of the steatosis biomarkers.
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Citation
Food and Function 2019,10, 2148-2160
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Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by/4.0/