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Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus)

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dc.contributor.authorSanguansook, P
dc.contributor.authorRodprasert, W
dc.contributor.authorSawangmake, C
dc.contributor.authorFerrán, JL
dc.contributor.authorSoria-Monzó, P
dc.contributor.authorChatdarong, K
dc.contributor.authorGimeno Arias, Lourdes
dc.contributor.authorIzquierdo Rico, María José
dc.contributor.authorAvilés Sánchez, Manuel
dc.contributor.departmentBiología Celular e Histología
dc.date.accessioned2025-01-28T07:59:05Z
dc.date.available2025-01-28T07:59:05Z
dc.date.issued2024-05
dc.description© 2024 Elsevier Inc. This document is the published version of a published work that appeared in final form in Theriogenology To access the final edited and published work see: https://doi.org/10.1016/j.theriogenology.2024.02.032
dc.description.abstractBecause of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives. This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively. Amplification for sequencing of feline IZUMO1 and JUNO was performed using the RT-PCR method. Levels of gene expression in different tissues were evaluated using real-time PCR. In situ hybridization was performed to localize JUNO mRNA in ovarian tissues. The complete coding sequences of IZUMO1 and JUNO were obtained and analyzed. A comparison between protein orthologs demonstrated the conservation of IZUMO1 and JUNO in Felidae. The real-time PCR results from various visceral organs indicated that IZUMO1 was significantly higher in the testis than in other organs, whereas JUNO was significantly higher in the ovary than in other organs. Expression of IZUMO1 was found to be higher in the testes than in the caput, corpus, and cauda of epididymides. In situ hybridization revealed that JUNO mRNA was in the ooplasm and nucleus of the primordial, primary, secondary, and antral follicles. Importantly, this was the first study to demonstrate the IZUMO1 and JUNO genes in the testis and ovary of cats. The results are useful for future research related to these genes and for developing contraceptives against these targets.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.citationTheriogenology Volume 220, May 2024, Pages 70-76
dc.identifier.doihttps://doi.org/10.1016/j.theriogenology.2024.02.032
dc.identifier.issnPrint.: 0093-691X
dc.identifier.issnElectronic.: 1879-3231
dc.identifier.urihttp://hdl.handle.net/10201/149387
dc.languageenges
dc.publisherElsevieres
dc.relationThis research project is supported by Second Century Fund (C2F), Chulalongkorn university and the 90th Anniversary of Chulalongkorn University Scholarship. This work is additionally supported by PID2021-123091NB-C21, funded by MCIN/ AEI /10.13039/501100011033/ and by FEDER Una manera de hacer Europa. Spanish Ministry of Science, Innovation and Universities (MCIU), State Research Agency (AEI) and European Regional Development Fund (FEDER) (PGC2018-098229-B-100) provided support to José Luis Ferran.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0093691X24001006?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFelis catus
dc.subjectIn situ hybridization
dc.subjectIZUMO1
dc.subjectJUNO
dc.subjectSequencing
dc.titleExpression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus)es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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relation.isAuthorOfPublicationeb2d72d3-a1d5-48cc-b71f-4aaf60a9aeac
relation.isAuthorOfPublication.latestForDiscoverya0170301-b64f-46f8-810a-ff18c9bf0a5f
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