Publication: Expression of IZUMO1 and JUNO in the gonads of domestic cats (Felis catus)
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Date
2024-05
Authors
Sanguansook, P ; Rodprasert, W ; Sawangmake, C ; Ferrán, JL ; Soria-Monzó, P ; Chatdarong, K ; Gimeno Arias, Lourdes ; Izquierdo Rico, María José ; Avilés Sánchez, Manuel
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.theriogenology.2024.02.032
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info:eu-repo/semantics/article
Description
© 2024 Elsevier Inc.
This document is the published version of a published work that appeared in final form in Theriogenology
To access the final edited and published work see:
https://doi.org/10.1016/j.theriogenology.2024.02.032
Abstract
Because of the time-consuming nature of surgical neutering and the rapid rate of reproduction among domestic
cats, it is crucial to investigate alternative, nonsurgical methods of contraception for this species. Sperm protein
IZUMO1 and its oocyte receptor JUNO have been proposed as potential targets for nonsurgical contraceptives.
This study aimed to demonstrate (1) the protein coding sequence of feline IZUMO1 and JUNO, (2) gene
expression in specific organs by measuring mRNA levels in different visceral tissues, and (3) the expression of
IZUMO1 and JUNO during sperm maturation and folliculogenesis, respectively. Amplification for sequencing of
feline IZUMO1 and JUNO was performed using the RT-PCR method. Levels of gene expression in different tissues
were evaluated using real-time PCR. In situ hybridization was performed to localize JUNO mRNA in ovarian
tissues. The complete coding sequences of IZUMO1 and JUNO were obtained and analyzed. A comparison between
protein orthologs demonstrated the conservation of IZUMO1 and JUNO in Felidae. The real-time PCR
results from various visceral organs indicated that IZUMO1 was significantly higher in the testis than in other
organs, whereas JUNO was significantly higher in the ovary than in other organs. Expression of IZUMO1 was
found to be higher in the testes than in the caput, corpus, and cauda of epididymides. In situ hybridization
revealed that JUNO mRNA was in the ooplasm and nucleus of the primordial, primary, secondary, and antral
follicles. Importantly, this was the first study to demonstrate the IZUMO1 and JUNO genes in the testis and ovary
of cats. The results are useful for future research related to these genes and for developing contraceptives against these targets.
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Citation
Theriogenology Volume 220, May 2024, Pages 70-76
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