Publication: Lectin-staining pattern in extratesticular rete testis and ductuli efferentes of prepubertal and adult horses
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Date
1998
Authors
Parillo, F. ; Stradaioli, G. ; Verini Supplizi, A. ; Monaci, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study was undertaken to determine the
lectin affinity of the extratesticular rete testis and ductuli
efferentes epithelial cells in adult and prepubertal horses.
using ten different lectin horseradish peroxidase
conjugates: Con-A, LCA, WGA, GSA-11, SBA, PNA,
RCA-1, DBA, UEA-1, and LTA. In some cases,
treatments with sialidase and KOH preceded the lectin
staining. In sexually mature and immature horses the
results showed the presence of different kinds of
sialoglycoconjugates with the terminal sialic acid linked
to D-GalNAc and B-D-Gal residues in the rete testis. In
the apical surface and cytoplasm of epithelial cells lining
the ductuli efferer~teso f the adult horse, glycoconjugates
with a-D-Man andlor a-D-Glc, GlcNAc, D-GalNAc and
B-D-Gal residues were evidenced, whereas in the
prepubertal horse only the apical surface of the ductuli
efferentes epithelial cells resulted reactive toward some
lectins. The differences observed in the presence of
glycoconjugates between adult and prepubertal horse
ductuli efferentes, suggest a hormonal control of the
function of these tracts of the post-testicular ducts.
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