Publication:
Use of digital image analysis to improve rigor and efficiency of physeal bone growth measurements

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dc.contributor.authorJay Byrd
dc.contributor.authorBria Foley
dc.contributor.authorAlexander Farell
dc.contributor.authorGarret Williams
dc.contributor.authorMax Twedt
dc.contributor.authorJames Sypherd
dc.contributor.authorEllen Leiferman
dc.contributor.authorCameron Jeffers
dc.contributor.authorMatthew A. Halanski
dc.contributor.authorGrant Ozaki
dc.contributor.departmentHistología e Histopatología
dc.coverage.spatialeast=NaN; north=NaN
dc.date.accessioned2025-09-25T10:34:55Z
dc.date.available2025-09-25T10:34:55Z
dc.date.issued2025
dc.description.abstractIn larger, translational animal models, manual measurements of longitudinal bone growth using fluorochrome labels is tedious and may be prone to less rigor due to variations in reader experience, sampling differences, and photobleaching that limits the repeatability of measurements. This study assesses the reliability of three different digital methods to assist in measurement of distance between pulsed fluorochrome labels. Forty-five tibial physes from skeletally immature New Zealand White rabbits were pulsed with fluorochrome labels and measured using Fully Manual Technique (FMT), Manual Digital Measurement (MDM), Computer Assisted Image Processing (AIP), and Fully Automated Measurement (FAM). Pearson’s correlation coefficient and Bland-Altman analysis were used to analyze the different methods. Intraclass correlation coefficient (ICC) assessed inter- and intrareader reliability. Regardless of the method, all growth rate measurement techniques exhibited excellent agreement and reliability. The computer assisted methods allowed rapid data acquisition without compromising reliability, thereby improving efficiency in bone growth research. Clinical Significance: Distances between fluorochrome bone labels on large samples can be reliably measured using digital imaging and processing techniques.
dc.formatapplication/pdf
dc.format.extent8
dc.identifier.citationHistology and Histopathology, Vol.40, nº9, (2025)
dc.identifier.doi10.14670/HH-18-875
dc.identifier.eissn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/161689
dc.languageeng
dc.publisherUniversidad de Murcia, Departamento Histología e Histopatología
dc.relationSin financiación externa a la Universidad
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.subjectBone Label
dc.subjectFluorochrome
dc.subjectOxytetracycline
dc.subjectAlizarin Red
dc.subjectGrowth Rate
dc.subject.odsNo relacionado con ningún objetivo de desarrollo sostenible
dc.titleUse of digital image analysis to improve rigor and efficiency of physeal bone growth measurements
dc.typeinfo:eu-repo/semantics/article
dspace.entity.typePublicationes
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Histology and histopathology Vol.40, nº9 (2025)