Publication: Use of digital image analysis to improve rigor and efficiency of physeal bone growth measurements
Authors
Jay Byrd ; Bria Foley ; Alexander Farell ; Garret Williams ; Max Twedt ; James Sypherd ; Ellen Leiferman ; Cameron Jeffers ; Matthew A. Halanski ; Grant Ozaki
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Publisher
Universidad de Murcia, Departamento Histología e Histopatología
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DOI
10.14670/HH-18-875
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info:eu-repo/semantics/article
Description
Abstract
In larger, translational animal models,
manual measurements of longitudinal bone growth using
fluorochrome labels is tedious and may be prone to less
rigor due to variations in reader experience, sampling
differences, and photobleaching that limits the
repeatability of measurements. This study assesses the
reliability of three different digital methods to assist in
measurement of distance between pulsed fluorochrome
labels. Forty-five tibial physes from skeletally immature
New Zealand White rabbits were pulsed with
fluorochrome labels and measured using Fully Manual
Technique (FMT), Manual Digital Measurement
(MDM), Computer Assisted Image Processing (AIP),
and Fully Automated Measurement (FAM). Pearson’s
correlation coefficient and Bland-Altman analysis were
used to analyze the different methods. Intraclass
correlation coefficient (ICC) assessed inter- and intrareader reliability. Regardless of the method, all growth
rate measurement techniques exhibited excellent
agreement and reliability. The computer assisted
methods allowed rapid data acquisition without
compromising reliability, thereby improving efficiency
in bone growth research. Clinical Significance:
Distances between fluorochrome bone labels on large
samples can be reliably measured using digital imaging
and processing techniques.
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Citation
Histology and Histopathology, Vol.40, nº9, (2025)
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