Publication: Methods for detecting apoptotic cells in tissues
Authors
Sanders, E.J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In this review, methods currently available
for the detection of cell death in tissues are surveyed,
with special reference to techniques that allow the
recognition of apoptotic cells in situ, either in whole
mount specimens or in tissue sections. The techniques
considered include: severa1 variations on in situ DNA
nick-end labelling methods, vital dyes, lysosomal
enzyme histochemistry, transglutaminase expression,
and immunocytochemical detection of severa1 deathassociated
antigens. These methods are discussed in
relation to their utility in detecting different stages of cell
death, and also to their ability to distinguish between
apoptotic and necrotic cell death.
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Citation
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