Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

Pelegrin, Pablo; García-Castillo, Jesús; Meseguer, José; Mulero Méndez, Victoriano Francisco

Publication:
Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines

dc.contributor.author	Pelegrin, Pablo
dc.contributor.author	García-Castillo, Jesús
dc.contributor.author	Meseguer, José
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2024-01-24T17:53:06Z
dc.date.available	2024-01-24T17:53:06Z
dc.date.issued	2001
dc.description	©2001. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Cytokine. To access the final edited and published work see https://doi.org/10.1006/cyto.2001.0949	es
dc.description.abstract	The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5’ untraslated region (UTR), a single open reading frame of a 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3’UTR with a polyadenilation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation.	es
dc.format	application/pdf	es
dc.format.extent	15	es
dc.identifier.citation	Cytokine, volumen 16, nº 2, año 2001, páginas 67-72.
dc.identifier.doi	10.1006/cyto.2001.0949
dc.identifier.issn	1043-4666
dc.identifier.uri	http://hdl.handle.net/10201/137725
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Spanish Ministry of Science and Technology (grant number PB98-0387) and Fundación Séneca.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S1043466601909494?via%3Dihub	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 Internacional	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Peces	es
dc.subject	Interleucina-1	es
dc.subject	Macrófagos	es
dc.subject	Filogenia	es
dc.subject.other	CDU::6 - Ciencias aplicadas	es
dc.title	Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
relation.isAuthorOfPublication	369f9ade-8896-4d91-84c5-e3f94a526070
relation.isAuthorOfPublication.latestForDiscovery	369f9ade-8896-4d91-84c5-e3f94a526070