Publication: Interleukin-1β isolated from a marine fish reveals up-regulated expression in macrophages following activation with lipopolysaccharide and lymphokines
Authors
Pelegrin, Pablo ; García-Castillo, Jesús ; Meseguer, José ; Mulero Méndez, Victoriano Francisco
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Publisher
Elsevier
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DOI
10.1006/cyto.2001.0949
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info:eu-repo/semantics/article
Description
©2001. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Cytokine. To access the final edited and published work see https://doi.org/10.1006/cyto.2001.0949
Abstract
The gilthead seabream IL-1β gene consists of five exons/four introns. The complete coding sequence contains a 102 bp 5’ untraslated region (UTR), a single open reading frame of a 762 bp which translates into a 253 amino acid molecule, and a 407 bp 3’UTR with a polyadenilation signal 14 nucleotides upstream of the poly(A)tail. The seabream sequence has highest degree of nucleotide (61.7%) and amino acid (53%) identity with the trout IL-1β sequences. The IL-1β message was detected by RT-PCR in head-kidney, blood, spleen, liver, gill and peritoneal exudate of both non-infected and Vibrio anguillarum-challenged fish. More importantly, IL-1β was highly expressed by purified macrophage monolayers and was up-regulated by lipopolysaccharide and lymphocyte-derived macrophage-activating factor stimulation.
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Citation
Cytokine, volumen 16, nº 2, año 2001, páginas 67-72.
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