Publication: The effect of bilberries on diabetes-related alterations of interstitial cells of Cajal in the lower oesophageal sphincter in rats
Authors
Velickov, Aleksandra ; Mitrovic, Olivera ; Djordjevic, Branka ; Sokolovic, Dusan ; Zivkovic, Vladimir ; Velickov, Asen ; Pantovic, Vukica ; Poklar Urlih, Natasa ; Radenkovic, Goran
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-11-829
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info:eu-repo/semantics/article
Description
Abstract
Diabetic gastroenteropathy involves not only
the parasympathetic and sympathetic autonomic nerves,
but also enteric neurons, smooth muscle cells and
interstitial cells of Cajal (ICC). ICC are the cells of
mesenchymal origin that occur within and around the
muscle layers in the gastrointestinal tract. The objective
of the present study was to investigate the alterations of
ICC in the lower oesophageal sphincter (LOS) of
streptozotocin-nicotinamide non-insulin-dependent
diabetes rats. Moreover, we investigated possible ICC in
rats with the same type of diabetes, treated with bilberry
fruit extract, bearing in mind that its hypoglycemic effect
had been already proven.
Male Wistar rats (10 weeks old) were used, and
diabetes was induced by an intraperitoneal injection of
streptozotocin, immediately after intraperitoneal
application of nicotinamide. The specimens were
exposed to anti-c-kit antibodies to investigate the
distribution of ICC, and the smooth muscle cells were
immunohistochemically labelled using anti-desmin
antibodies.
Intramuscular ICC were very abundant in the LOS
of rats. They were spindle-shaped, with two long
processes connecting them into long linear sequences. In
the LOS of diabetic rats, intramuscular ICC were rarely
present and linear cell-cell connections between these
cells were completely missing. In groups treated with
bilberry, the number and distribution of ICC were
exactly the same as in the above described rats with
induced diabetes.
In summary, a decrease of intramuscular ICC,
discontinuities and breakdown of contacts between ICC
were observed in streptozotocin-nicotinamide induced
diabetes rats and in groups treated with bilberry. Bilberry
fruit extract was shown to have hypoglycemic activity,
but without any protective effects on ICC in the LOS of
diabetic rats.
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Citation
Histology and Histopathology, Vol.32, nÂş6, (2017)
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