Publication: Cavernomas of the human brainstem:
3-dimensional reconstruction from histological
slides using computerized techniques
Authors
Toralba, Simon ; Landemore, Gérard ; Coquemont, Maëlle ; Plancoulaine, Benoit ; Chapon, Françoise
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Cerebral cavernous malformations (CCMs)
are described as vascular lesions consisting of
endothelial-lined dilated vessels embedded in a
connective tissue sheath without intervening
parenchyma between them.
Their anatomical connections with the normal blood
vessels are still enigmatic and the fine three-dimensional
(3-D) organization of these vascular lesions remains to
be established.
Two stacks of serial histological slices, obtained
from two brainstem CCM lesions (from the necropsy of
a CCM2 male patient), were stained using Masson’s
trichrome method and then digitized. Stacks of regions
of interest underwent quasi-automatic processing: 1)
propagative registering using blockmatching algorithms
and Brain Visa programs; 2) 3-D segmentation using
Aphelion; 3) display with Anatomist or ImageVis3D.
These first histological 3-D reconstructions show the
external limits of the caverns defined as the external
limit of their collagen sheath.
These pictures not only reveal the gross spatial
organization of the lesions, but due to their high
resolution (4 µm) and with the help of simple anaglyphic
3-D rendering, they also allow the visualization of
connections between caverns and very small blood
vessels.
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Citation
Histology and Histopathology, vol. 29, nº 8, (2014)
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