Publication: The influence of immunosuppressants on the
morphology, proliferating cell nuclear antigen
(PCNA) and apoptosis in the rat ventral prostate
Authors
Grabowska, Marta ; Słuczanowska-Głąbowska, Sylwia ; Kram, Andrzej ; Teresiński, Leszek ; Piasecka, Małgorzat ; Podhorska-Okołów, Marzenna ; Rotter, Iwona ; Kędzierska, Karolina ; Sporniak-Tutak, Katarzyna ; Ciechanowski, Kazimierz ; Laszczyńska, Maria
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14570/HH-11-606
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info:eu-repo/semantics/article
Description
Abstract
Aim: Analysis of the impact of immunosuppressants on apoptosis and PCNA in the rat ventral
prostate. Method: The studies were performed on 48
male Wistar rats. The animals were divided into a
control group and 7 experimental groups. For 6 months,
the rats were administered drugs such as: rapamycin
(Rapa), cyclosporin A (CsA), tacrolimus (Tac),
mycophenolate mofetil (MMF) and glucocorticosteroids
(GS). During section of the rats, prostate ventral lobes
were obtained. Morphological evaluation (HE, PAS),
TUNEL assay, PCNA expression analysis and
quantitative image computer analysis were performed.
Results: The highest percentage of apoptosis in epithelial
cells was observed in groups which received two
combinations of drugs: (V) CsA, MMF, GS and (VII)
Tac, MMF, GS. A much lower percentage of apoptotic
epithelial cells was found in the groups where the
treatment schemes included rapamycin throughout the
duration of the study. Interestingly, the conversion of the
treatment to rapamycin caused a significant reduction of
apoptosis in epithelial cells as well as in proliferation in
both epithelial and stromal cells. Conclusions: On one
hand, the obtained results may explain the anticancer activity of rapamycin in reducing the proliferation of
epithelial cells, and on the other hand the adverse effect
of rapamycin in the form of reduced regeneration of
these cells. Taking into account the prostate in isolation
from other organs/systems, the dosage scheme with
Rapa, Tac and GS would appear to be the most
favorable, due to the smallest morphological changes.
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Citation
Histology and histopathology, Vol. 30, nº 9 (2015)
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