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Extracellular vesicles would be involved in the release and delivery of seminal TGF-β isoforms in pigs

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dc.contributor.authorPadilla, Lorena
dc.contributor.authorBarranco, Isabel
dc.contributor.authorParra, Ana
dc.contributor.authorParrilla, Inmaculada
dc.contributor.authorRodríguez Martínez, Heriberto
dc.contributor.authorLucas, Xiomara
dc.contributor.authorRoca, Jordi
dc.contributor.authorMartínez Hernández, Jesús
dc.contributor.authorPastor García, Luis Miguel
dc.contributor.departmentMedicina y Cirugía Animal
dc.date.accessioned2024-09-05T09:58:09Z
dc.date.available2024-09-05T09:58:09Z
dc.date.issued2023-02-10
dc.description© 2023 Padilla, Barranco, Martínez-Hernández, Parra, Parrilla, Pastor, Rodriguez-Martinez, Lucas and Roca. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by/4.0/. This document is the Published version of a Published Work that appeared in final form in Frontiers in Veterinary Science. To access the final edited and published work see https://doi.org/10.3389/fvets.2023.1102049
dc.description.abstractIntroduction: pig seminal plasma (SP) is rich in active forms of all three isoforms (1-3) of transforming growth factor β (TGF-β), a chemokine modulatory of the immune environment in the female genital tract once semen is delivered during mating or artificial insemination (AI). The present study aimed to examine how TGF-βs are secreted by the epithelium of the male reproductive tract and how they are transported in semen, emphasizing the interplay with seminal extracellular vesicles (sEVs). Methods: Source of TGF-βs was examined by immunohistochemistry in testis, epididymis, and accessory sex glands, by immunocytochemistry in ejaculated spermatozoa, and by Luminex xMAP® technology in SP and sEVs retrieved from healthy, fertile male pigs used as breeders in AI programs. Results: All three TGF-β isoforms were expressed in all reproductive tissues explored and would be released into ductal lumen either in soluble form or associated with sEVs. Ejaculated spermatozoa expressed all three TGF-β isoforms, both inside and outside, probably the outer one associated with membrane-bound sEVs. The results confirmed that pig SP contains all three TGF-β isoforms and demonstrated that a substantial portion of them is associated with sEVs. Discussion: Seminal EVs would be involved in the cellular secretion of the active forms of seminal TGF-β isoforms and in their safe transport from the male to the female reproductive tract.es
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dc.identifier.citationFront. Vet. Sci. 10:1102049
dc.identifier.doihttps://doi.org/10.3389/fvets.2023.1102049
dc.identifier.issnElectronic: 2297-1769
dc.identifier.urihttp://hdl.handle.net/10201/143606
dc.languageenges
dc.publisherFrontiers Media
dc.relationThis study was funded by the Spanish Ministry of Science and Innovation (grant PID2020-113493RB-I00 funded by MCIN/AEI/10.13039/501100011033), Madrid, Spain; the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 891382; and FORMAS, Stockholm (grant 2019-00288).es
dc.relation.publisherversionhttps://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1102049/fulles
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectExtracellular vesicleses
dc.subjectPorcine
dc.subjectSemen
dc.subjectSeminal plasma
dc.subjectSperm
dc.subjectTGFβ isoforms
dc.titleExtracellular vesicles would be involved in the release and delivery of seminal TGF-β isoforms in pigses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicatione442a579-c2af-4fdd-8e39-00e5581604cb
relation.isAuthorOfPublication920d1ad2-1252-4ca8-9187-f3a351c89ce2
relation.isAuthorOfPublication.latestForDiscoverye442a579-c2af-4fdd-8e39-00e5581604cb
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