Publication: Upregulation of miRNA-1228-3p alleviates TGF-β-induced fibrosis in renal tubular epithelial cells
Authors
Shen, Huajuan ; He, Qiang ; Dong, Yongze ; Shao, Lina ; Liu, Yueming ; Gong, Jianguang
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-242
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info:eu-repo/semantics/article
Description
Abstract
Background. Chronic kidney disease (CKD)
has become a major public health issue, which can lead
to renal fibrosis regardless of the initial injury. It has
been previously reported that miRNA-1228-3p was
correlate with the progression of kidney fibrosis.
However, the mechanism by which miRNA-1228-3p
regulates renal fibrosis remains unclear. Methods. Renal
tubular epithelial cells (HK-2) were treated with TGF-β1
(10 ng/ml) in an in vitro model of renal fibrosis. Gene
and protein expressions in HK-2 cells were measured by
Western-blot and RT-qPCR, respectively. The relation
between miRNA-1228-3p and its target gene was
investigated by dual luciferase report analysis. Results.
Upregulation of miRNA-1228-3p significantly inhibited
TGF-β1-induced fibrosis of HK-2 cells in vitro by
targeting GDF11. In addition, miRNA-1228-3p
exhibited anti-fibrosis effect through inhibition of the
smad2/smad4 signaling pathway. Conclusion.
Upregulation of miRNA-1228-3p markedly inhibited the
progression of renal fibrosis in vitro, indicating that
miRNA-1228-3p may serve as a potential novel target
for the treatment of renal fibrosis
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Citation
Histology and Histopathology Vol. 35, nº10 (2020)
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