Publication:
Lectin cytochemistry on developing rat submandibular gland primary cultures

Simple item page
dc.contributor.authorSabbieti, M.G.es
dc.contributor.authorGabrielli, M.G.es
dc.contributor.authorMenghi, Giovanna
dc.contributor.authorMaterazzi, G.
dc.contributor.authorMarchetti, L.
dc.date.accessioned2011-06-08T09:08:42Z
dc.date.available2011-06-08T09:08:42Z
dc.date.issued2004
dc.description.abstractLectin cytochemistry was performed in vitro on primary cultures from the rat submandibular gland. For this purpose, prepubertal rats (17, 27, 33 days old) of both sexes were used. Several types of medium supplements were tested and it was found that cells survived until 15 days in presence of all medium supplements and extracellular matrix gel. The binding patterns of all FITC/TRITC-labeled lectins, with and without prior sialidase digestion and deacetylation, were analyzed in a confocal laser scanning microscope. In particular, the occurrence of C4 acetylated sialic acid linked to ß-galactose at day 27 and the presence of fucose residues at day 33 indicated that lectin probes applied to cultured cells give results similar to those obtained in intact tissues and can be used as markers of growth and differentiation.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/21579
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectLectinses
dc.subjectSubmandibular glandes
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleLectin cytochemistry on developing rat submandibular gland primary cultureses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Lectin cytochemistry on developing.pdf
Size:
6.46 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.19, nº 3 (2004)