Publication: Lectin cytochemistry on developing rat submandibular gland primary cultures
Authors
Sabbieti, M.G. ; Gabrielli, M.G. ; Menghi, Giovanna ; Materazzi, G. ; Marchetti, L.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Lectin cytochemistry was performed in vitro
on primary cultures from the rat submandibular gland.
For this purpose, prepubertal rats (17, 27, 33 days old) of
both sexes were used. Several types of medium
supplements were tested and it was found that cells
survived until 15 days in presence of all medium
supplements and extracellular matrix gel. The binding
patterns of all FITC/TRITC-labeled lectins, with and
without prior sialidase digestion and deacetylation, were
analyzed in a confocal laser scanning microscope. In
particular, the occurrence of C4 acetylated sialic acid
linked to ß-galactose at day 27 and the presence of
fucose residues at day 33 indicated that lectin probes
applied to cultured cells give results similar to those
obtained in intact tissues and can be used as markers of
growth and differentiation.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.