Publication: Sites of lymph follicle formation in the draining popliteal lymph nodes of mice locally injected with antigenic and mitogenic substances
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Date
1990
Authors
Hee Kyung Ahnl ; Hajime Hoshi ; Kaeko Horie ; Hidetsugu Nagata
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Our previous studies showed that some
antigenic and mitogenic substances, when locally
injected into mice, efficiently produced new lymph
follicles outside pre-exisiting follicles in draining lymph
nodes, whereas others had virtually no effect. In the
present experiments, young adult male mice were
injected with several antigens and mitogens in the rear
footpad, and the number and development sites of newly
produced lymph follicles in the draining popliteal nodes
were studied using serial sections of the nodes obtained
between 5 and 21 days after injection.
In the unstimulated state, each popliteal node
contained a limited number of lymph follicles which
mostly lay in a portion of the peripheral cortex
overlaying the deep cortex (this portion is referred
to as the PCOU), whereas a portion of the peripheral
cortex extending beyond the deep cortex (referred to
as the PCBU) was underdeveloped with only occasional
follicles. Mice treated with soluble PHA or fluid tetanus
toxoid developed germinal centers in association with
existing follicles but failed to produce new follicles. The
PCBU of the draining nodes remained underdeveloped,
and the number and distribution pattern of lymph
follicles within a draining node were comparable to those
in the control node. Animals treated with LPS (50 pg). Con A, alum-precipitated PHA or alum-precipitated
tetanus toxoid produced significantly large numbers of
new follicles outside pre-existing follicles in the draining
nodes, the new follicles produced in the PCBU being
generally more numerous than those in the PCOU. In
these draining nodes, the peripheral cortex, comprising
a number of follicles, was found to overlie the deep
cortex and extend beyond the deep cortex towards the
hilar region. In animals given a less effective stimulant,
such as ferritin or a smaller dose of LPS (10 pg), the
draining nodes produced a relatively small number of
new follicles, most of which were formed in the PCBU. The present results indicate that in the mouse popliteal
node, the PCBU is morphologically underdeveloped
under normal conditions. but develops lymph follicles in
response to exogenous stimuli more readily than the
PCOU, and that substances efficient in inducing follicle
formation can be regarded as capable of stimulating the
development of the peripheral cortex.
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