Publication: Membrane skeletons in avian erythrocytes as revealed by the quick-freezing and deep-etching method
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Date
1997
Authors
Terada, N. ; Fuji, Y. ; Kitano, K. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Ultrastructure of chicken erythrocytes were
examined by the quick-freezing and deep-etching (QFDE)
method. Some erythrocytes were fixed with
paraformaldehyde and prepared with erythrocytesplitting
method or saponin treatment to remove soluble
proteins before quick-freezing. Others were prepared in
the cytosol buffer with the erythrocyte-splitting method
to obtain natural state of cytoskeletons. Non-expanding
membrane skeletons were highly condensed on the
cytoplasmic side of lipid membrane in the paraformaldehyde-
fixed specimens. Under unilateral
extension of the specimens, long stretched filaments
were connected alternately with condensed filamentous
or granular structures under erythrocyte membranes. As
the membrane skeletons got closer to the marginal
bands, they become more dense network structures.
Moreover, in the fresh unfixed specimens, dense
networks of filaments were localized underlying
erythrocyte membranes in a relatively intact state. Fine
filaments connected the marginal microtubule bands to
the cytoplasmic sides of erythrocyte membranes. The
different distribution of each cytoskeletal component and
the association of these structures may support the
elliptocytic shape of chicken erythrocytes and resist the
dynamic circumstance.
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