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A modified chemical protocol of decellularization of rat sciatic nerve and its recellularization with mesenchymal differentiated Schwann-Like cells: Morphological and functional assessments

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dc.contributor.authorGarcía Pérez, M.M.
dc.contributor.authorMartínez Rodríguez, H.G.
dc.contributor.authorLópez Guerra, G.G.
dc.contributor.authorSoto Domínguez, A.
dc.contributor.authorSaid Fernández, S.L.
dc.contributor.authorMorales Ávalos, R.
dc.contributor.authorElizondo Omaña, R.E.
dc.contributor.authorMontes de Oca Luna, R.
dc.contributor.authorGuzmán López, S.
dc.contributor.authorCastillo Galván, M.L.
dc.contributor.authorMendoza Lemus, O.F.
dc.contributor.authorVílchez Cavazos, F.
dc.date.accessioned2022-02-28T07:42:25Z
dc.date.available2022-02-28T07:42:25Z
dc.date.issued2017
dc.description.abstractThe functional reconstruction of large neural defects usually requires the use of peripheral nerve autografts, though these have certain limitations. As a result, interest in new alternatives for autograft development has risen. The acellular peripheral nerve graft is an alternative for peripheral nerve injury repair, but to date there is not a standardized chemical decellularization method widely accepted. The objective of this study was to propose a modified chemical protocol of decellularization of rat sciatic nerve and its recellularization in vitro with mesenchymal differentiated Schwann-like cells. After the transplantation, an evaluation of its regeneration was performed using morphological and functional tests. The study consisted of two phases; in phase 1, different concentrations and times of exposure of rat sciatic nerves to detergents were tested, to establish a modified chemical protocol for nerve decellularization. The chemical treatment with 3% triton X-100 and 4% sodium deoxycholate for 15 days allowed a complete decellularization whilst conserving the extracellular matrix of the harvested nerve. In phase 2, the decellularized and recellularized alografts were compared against autografts. The morphological analysis showed a higher positivity to specific myelin antibodies in the recellularized group compared to the autograft. There were no differences in this parameter between the control limb and the experimental limb (recellularized group). The functional analysis showed no statistical differences at week 15 in the Sciatic Function Index in the autograft group vs the other groups. This study sets the morphological and functional bases for posterior studies about nerve defects regeneration in humans.es
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dc.format.extent14es
dc.identifier.citationHistology and Histopathology, Vol.32, nº8, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-844
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117423
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAcellular graftes
dc.subjectDecellularizationes
dc.subjectPeripheral nervees
dc.subjectSchwann-like cellses
dc.subjectSciatic nervees
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleA modified chemical protocol of decellularization of rat sciatic nerve and its recellularization with mesenchymal differentiated Schwann-Like cells: Morphological and functional assessmentses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.32, nº8 (2017)