Publication: A modified chemical protocol of decellularization of rat sciatic nerve and its recellularization with mesenchymal differentiated Schwann-Like cells: Morphological and functional assessments
Authors
García Pérez, M.M. ; Martínez Rodríguez, H.G. ; López Guerra, G.G. ; Soto Domínguez, A. ; Said Fernández, S.L. ; Morales Ávalos, R. ; Elizondo Omaña, R.E. ; Montes de Oca Luna, R. ; Guzmán López, S. ; Castillo Galván, M.L. ; Mendoza Lemus, O.F. ; Vílchez Cavazos, F.
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-844
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info:eu-repo/semantics/article
Description
Abstract
The functional reconstruction of large neural
defects usually requires the use of peripheral nerve
autografts, though these have certain limitations. As a
result, interest in new alternatives for autograft
development has risen. The acellular peripheral nerve
graft is an alternative for peripheral nerve injury repair,
but to date there is not a standardized chemical
decellularization method widely accepted. The objective
of this study was to propose a modified chemical
protocol of decellularization of rat sciatic nerve and its
recellularization in vitro with mesenchymal differentiated
Schwann-like cells. After the transplantation, an
evaluation of its regeneration was performed using
morphological and functional tests. The study consisted
of two phases; in phase 1, different concentrations and
times of exposure of rat sciatic nerves to detergents were
tested, to establish a modified chemical protocol for
nerve decellularization. The chemical treatment with 3%
triton X-100 and 4% sodium deoxycholate for 15 days
allowed a complete decellularization whilst conserving
the extracellular matrix of the harvested nerve. In phase
2, the decellularized and recellularized alografts were
compared against autografts. The morphological analysis
showed a higher positivity to specific myelin antibodies
in the recellularized group compared to the autograft.
There were no differences in this parameter between the
control limb and the experimental limb (recellularized
group). The functional analysis showed no statistical
differences at week 15 in the Sciatic Function Index in
the autograft group vs the other groups. This study sets
the morphological and functional bases for posterior
studies about nerve defects regeneration in humans.
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Citation
Histology and Histopathology, Vol.32, nº8, (2017)
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