Publication: An evaluation of the role of nuclear cytoplasmic ratios and nuclear volume densities as diagnostic indicators in metaplastic, dysplastic and neoplastic lesions of the human cheek
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Date
1997
Authors
White, F.H. ; Jin, Y. ; Yang, Ling
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The increase in nuclear cytoplasmic (NIC)
ratio is one of the features of cellular atypia which is
used in the histopathological assessment of premalignant
lesions of the oral mucosa. Since this feature is readily
quantifiable using morphometry, we have analysed both
N/C and nuclear volume densities in basal and spinous
cells from human cheek lesions with and without
malignant potential in order to ascertain the validity
of this parameter as a predictor. Using a strictly
standardised sampling procedure, measurements of
cellular and nuclear areas of basal and spinous cells from
normal and pathological human cheek mucosa were
made on haematoxylin and eosin-stained sections using
a VIDAS image analyser. Cases examined comprised
fibrous hyperplasia (FH), traumatic inflammation (IF),
benign hyperkeratosis (HK), lichen planus (LI),
leukoplakia with dysplasia (DYS), squamous cell
papilloma (PP), dysplastic epithelium from the edges
adjacent to invasive carcinoma (CE) and islands from
invasive squamous cell carcinoma (CI). In basal cells,
NIC ratios and nuclear volume densities were lower than
values obtained for the normal controls. In spinous cells,
these parameters were elevated in the potentially premalignant lesions (DYS, CE) as well as in CI but
values were similarly elevated in FH, IF, HK and PP,
lesions which appear to have no malignant potential.
The NIC ratio is of no value as a predictor of
malignant potential in basal or spinous cells from cheek
lesions. The putative increase in NIC which has been
previously described qualitatively is probably due to
increased nuclear hyperchromatism, which may provide
an illusory increase in relative nuclear size at the
expense of the cytoplasm.
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