Publication: Multiparameter analysis of AgNOR in thyroid lesions: comparison with PCNA expression
Authors
Slowinska-Klencka, D. ; Klencki, M. ; Popowicz, B. ; Sporny, S. ; Lewinski, A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the study was to examine
numerous features of argyrophilic proteins related to
nucleolar organizer regions (AgNORs) in thyroid
tumors, relate them to PCNA expression and evaluate
which of these features might be useful in the diagnosis
of thyroid lesions. Paraffin sections of 100 thyroid
tumors were silver-stained and divided into 9 groups:
nodular goiter (NG), simple adenoma (SA),
microfollicular adenoma (MFA), follicular carcinoma
(FC), follicular variant of papillary carcinoma (PC-F),
classical variant of papillary carcinoma (PC-C), Hürthle
cell adenoma (HA), Hürthle cell carcinoma (HC), and
anaplastic carcinoma (AC). The slides were analyzed
with the computerized system for image analysis. A
weak correlation was found between PCNA expression
and AgNOR size. AC differed significantly from all
other examined groups in many features of AgNOR
dots. Hürthle cell neoplasms were characterized by the
presence of a usually single and relatively large dot.
With respect to diagnosing follicular lesions, we found
that the evaluation of the total area of dots in the nucleus
seemed to be the most useful for discrimination: the
assumption of 4.9 µm2, as a cut-off value, allowed a
correct classification of 77% of FC cases. Computeraided
morphometric analysis of AgNORs may be useful
in the diagnostics of thyroid lesions.
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