The colony-stimulating factor-1 receptor is a specific marker of macrophages from the bony fish gilthead seabream

Lopez-Castejón, Gloria; Meseguer Peñalver, J.; Mulero Méndez, Victoriano Francisco; Sepulcre Cortés, María Pilar; Roca Soler, Francisco José

Publication:
The colony-stimulating factor-1 receptor is a specific marker of macrophages from the bony fish gilthead seabream

dc.contributor.author	Lopez-Castejón, Gloria
dc.contributor.author	Meseguer Peñalver, J.
dc.contributor.author	Mulero Méndez, Victoriano Francisco
dc.contributor.author	Sepulcre Cortés, María Pilar
dc.contributor.author	Roca Soler, Francisco José
dc.contributor.department	Bioquímica y Biología Molecular B e Inmunología
dc.date.accessioned	2025-01-29T16:34:09Z
dc.date.available	2025-01-29T16:34:09Z
dc.date.issued	2005-08-30
dc.description	© 2005 Elsevier Ltd. All rights reserved.This document is the Published version of a Published Work that appeared in final form in Molecular Immunology. To access the final edited and published work see https://doi.org/10.1016/j.molimm.2005.07.028
dc.description.abstract	We report the molecular cloning of the colony-stimulating factor-1 receptor gene from the bony fish gilthead seabream (sbCSF-1R). The deduced sbCSF-1R shows a predicted signal sequence, a transmembrane domain and a tyrosine kinase domain, all in conserved positions. A transcript showing a premature stop codon that predicted the removal of 84 C-terminal amino acids was also found. RT-PCR expression studies demonstrate that, although the sbCSF-1R transcripts are found in different immune tissues, including gill, liver, spleen, blood, peritoneal exudate, thymus and head-kidney (HK), their expression is confined to the monocyte/macrophage lineage. Furthermore, the expression of sbCSF-1R might be modulated by the activation stage of the macrophages, since both the infection of fish and the in vitro activation of leukocytes resulted in the down-regulation of gene expression. These data indicate that the CSF-1R may be used as a specific probe for cells of the monocyte/macrophage lineage in the gilthead seabream, an immunological tractable fish model. In addition, the functional characterisation of the CSF-1R and its ligand may shed light into the mechanisms of proliferation and the pathways of differentiation of macrophages in bony fish.	es
dc.format	application/pdf	es
dc.format.extent	6	es
dc.identifier.citation	Molecular Immunology 43 (2006) 1418–1423
dc.identifier.doi	https://doi.org/10.1016/j.molimm.2005.07.028
dc.identifier.issn	Print: 0161-5890
dc.identifier.issn	Electronic: 1872-9142
dc.identifier.uri	http://hdl.handle.net/10201/149667
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	Spanish Ministry of Education and Science (grants BIO2001-2324-C02-02, AGL2002-03529 and AGL2002-04306-C03-01 to V.M. and J.M. and fellowship to G.L.-C.) and Fundación Séneca, Regional Agency for Science and Technology (grant PI-51/00782/FS/01 to J.M.)	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S016158900500307X?via%3Dihub
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	CSF1R	es
dc.subject	Macrophage marker	es
dc.subject	Phylogeny
dc.subject	Fish
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología	es
dc.title	The colony-stimulating factor-1 receptor is a specific marker of macrophages from the bony fish gilthead seabream	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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