Publication: The colony-stimulating factor-1 receptor is a specific marker of macrophages from the bony fish gilthead seabream
Authors
Lopez-Castejón, Gloria ; Meseguer Peñalver, J. ; Mulero Méndez, Victoriano Francisco ; Sepulcre Cortés, María Pilar ; Roca Soler, Francisco José
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.molimm.2005.07.028
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info:eu-repo/semantics/article
Description
© 2005 Elsevier Ltd. All rights reserved.This document is the Published version of a Published Work that appeared in final form in Molecular Immunology. To access the final edited and published work see https://doi.org/10.1016/j.molimm.2005.07.028
Abstract
We report the molecular cloning of the colony-stimulating factor-1 receptor gene from the bony fish gilthead seabream (sbCSF-1R). The deduced sbCSF-1R shows a predicted signal sequence, a transmembrane domain and a tyrosine kinase domain, all in conserved positions. A transcript showing a premature stop codon that predicted the removal of 84 C-terminal amino acids was also found. RT-PCR expression studies demonstrate that, although the sbCSF-1R transcripts are found in different immune tissues, including gill, liver, spleen, blood, peritoneal exudate, thymus and head-kidney (HK), their expression is confined to the monocyte/macrophage lineage. Furthermore, the expression of sbCSF-1R might be modulated by the activation stage of the macrophages, since both the infection of fish and the in vitro activation of leukocytes resulted in the down-regulation of gene expression. These data indicate that the CSF-1R may be used as a specific probe for cells of the monocyte/macrophage lineage in the gilthead seabream, an immunological tractable fish model. In addition, the functional characterisation of the CSF-1R and its ligand may shed light into the mechanisms of proliferation and the pathways of differentiation of macrophages in bony fish.
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Citation
Molecular Immunology 43 (2006) 1418–1423
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