Publication:
Sudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sections

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dc.contributor.authorOliveira, V.C.
dc.contributor.authorCarrara, R.C.V.
dc.contributor.authorSimoes, D.L.C.
dc.contributor.authorSaggioro, F.P.
dc.contributor.authorCarlotti, C.G.
dc.contributor.authorCovas, D.T., Jr.
dc.contributor.authorNeder, L.
dc.date.accessioned2015-10-13T14:40:01Z
dc.date.available2015-10-13T14:40:01Z
dc.date.issued2010
dc.description.abstractInterference by autofluorescence is one of the major concerns of immunofluorescence analysis of in situ hybridization-based diagnostic assays. We present a useful technique that reduces autofluorescent background without affecting the tissue integrity or direct immunofluorescence signals in brain sections. Using six different protocols, such as ammonia/ethanol, Sudan Black B (SBB) in 70% ethanol, photobleaching with UV light and different combinations of them in both formalin-fixed paraffin-embedded and frozen human brain tissue sections, we have found that tissue treatment of SBB in a concentration of 0.1% in 70% ethanol is the best approach to reduce/eliminate tissue autofluorescence and background, while preserving the specific fluorescence hybridization signals. This strategy is a feasible, non-time consuming method that provides a reasonable compromise between total reduction of the tissue autofluorescence and maintenance of specific fluorescent labels.es
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/46603
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 25, nº 8 (2010)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHuman braines
dc.subjectAutofluorescencees
dc.subject.other616 - Patología. Medicina clínica. Oncologíaes
dc.titleSudan Black B treatment reduces autofluorescence and improves resolution of in situ hybridization specific fluorescent signals of brain sectionses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.25, nº8 (2010)