Publication: Efectos biológicos de cuatro cementos definitivos usados en implantología sobre los fibroblastos gingivales humanos
Authors
Guerrero Gironés, José Luis
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Escuela Internacional de Doctorado
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Guerrero Gironés, Julia ; Pecci Lloret, María Pilar
Publisher
Universidad de Murcia
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DOI
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info:eu-repo/semantics/doctoralThesis
Description
Abstract
Objetivo: Determinar los efectos biológicos de cuatro cementos; RelyxUnicem 2 (RU), Panavia V5 (PV5), Multilink Hybrid Abutment (MHA) y SoloCem (SC) en fibroblastos gingivales humanos.
Material y métodos: Fibroblastos gingivales humanos (FGH) se expusieron a diferentes eluatos de cementos a base de resina. El efecto citotóxico y la migración celular se evaluaron utilizando los ensayos MTT y de cicatrización de herida. La adhesión y la morfología celular se analizaron mediante microscopía electrónica de barrido (MEB). Se determinaron los niveles de especies reactivas del oxígeno intracelular (ROS) producidos por los cementos. El estudio estadístico se realizó utilizando un análisis de varianza unidireccional (ANOVA) y la prueba de Tukey (p <0,05).
Resultados: MHA no afectó la viabilidad celular en las primeras 24h o 72h con cualquier dilución, y mostró diferencias significativas en comparación con el control (**p<0,01) a las 48h sin diluir y con la dilución 1:2. Los extractos de SC sin diluir (1:1) provocaron una disminución de la viabilidad de los FGH en todos los tiempos de estudio con diferencias significativas en comparación con el grupo control (***p<0,0001). Igualmente, la migración celular se vio afectada en presencia del grupo de SC sin diluir en todos los períodos en comparación con el control (***p<0,001). Por el contrario, en todos los tiempos de estudio y en todas las diluciones estudiadas, las tasas de migración celular en el grupo MHA fueron similares a las del grupo control. En relación a la adhesión celular, MEB mostró muy pocas células en el grupo SC.
Conclusiones: Los eluatos de MHA mostraron una mejor migración celular, mayor adhesión celular y mayor viabilidad celular que los obtenidos con los otros cementos. El grupo SC mostró peores resultados en todos los parámetros.
Objective: To determinate the biological effects of four cements; RelyxUnicem 2 (RU), Panavia V5 (PV5), Multilink Hibryd Abutment (MHA) and SoloCem (SC) on human gingival fibroblasts. Materials and Methods: Human gingival fibroblasts (HGF) were exposed to different eluates of resin-based cements. The cytotoxic effect and cell migration were assessed using MTT and wound-healing assays. The attachment and the cell morphology were analyzed by Scanning Electron Microscopy (SEM). The levels of intracellular reactive oxygen species (ROS) produced by the eluates of the different cements were determined. Data from results were performed using a one-way analysis of variance (ANOVA) and Turkey’s test (p< 0.05). Results: MHA did not affect cell viability in the first 24h or 72h at any dilution, and it showed significant differences in comparison to the control (**p<0.01) at 48h undiluted and 1:2 dilution. Extracts of SC reduces the viability of HGFs at any time undiluted with significant differences in comparison to the control (***p<0.0001).Cell migration was affected by the undiluted SC group at all period-times compared to the control (***p<0.001). Contrarily, at all period-times and all dilutions studied, cell migration rates in the MHA group were similar to that of the control group. SEM showed very few cells in SC group. Conclusions: MHA eluates showed a better cell migration, greater cell attachment and higher cell viability than those obtained with the other cements. SC group showed worse results in all parameters.
Objective: To determinate the biological effects of four cements; RelyxUnicem 2 (RU), Panavia V5 (PV5), Multilink Hibryd Abutment (MHA) and SoloCem (SC) on human gingival fibroblasts. Materials and Methods: Human gingival fibroblasts (HGF) were exposed to different eluates of resin-based cements. The cytotoxic effect and cell migration were assessed using MTT and wound-healing assays. The attachment and the cell morphology were analyzed by Scanning Electron Microscopy (SEM). The levels of intracellular reactive oxygen species (ROS) produced by the eluates of the different cements were determined. Data from results were performed using a one-way analysis of variance (ANOVA) and Turkey’s test (p< 0.05). Results: MHA did not affect cell viability in the first 24h or 72h at any dilution, and it showed significant differences in comparison to the control (**p<0.01) at 48h undiluted and 1:2 dilution. Extracts of SC reduces the viability of HGFs at any time undiluted with significant differences in comparison to the control (***p<0.0001).Cell migration was affected by the undiluted SC group at all period-times compared to the control (***p<0.001). Contrarily, at all period-times and all dilutions studied, cell migration rates in the MHA group were similar to that of the control group. SEM showed very few cells in SC group. Conclusions: MHA eluates showed a better cell migration, greater cell attachment and higher cell viability than those obtained with the other cements. SC group showed worse results in all parameters.
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