Publication: Effects of glia-conditioned medium on primary cultures
of central neurons
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Date
1989
Authors
Conde Guerri, B. ; Sinués Porta, E. ; Arrazola Schlamilch, M. ; Comuñas González, F. ; Maldonado, V. Calatayud
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effects of glia conditioned media on the
survival and differentiation of embryonic neuron
cultures of the Central Nervous System is described. We
established glial cultures of peritumoral areas and the
culture medium was changed serially and collected as glia
conditioned medium (GCM). Neuron enriched cultures
plated on poly-L-lysine coated Petri dishes and after 4
days in vitro. the neuronal cultures were treated for the
inhibition of glial cells. We established two
groups of neuronal cultures that were respectively exposed
to the GCM and to conventional culture medium. We
evaluated the survival and differentiation of neuronal
population in each group of cultures by contrast-phase
microscopy and cellular uptake of Horse Radish
Peroxidase. The cell cultures exposed to GCM showed a
survival during the in vitro stages and an organization
and differentiation pattern of mature neurons larger than
the control cultures. This fact suggests that the glial
cultures produce a diffusible neurotrophic factor that
influences the neuronal response in vitro.
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