Publication: Histochemical study of glycoconjugates in the toadfish Halobatrachus didactylus oesophagus epithelium
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Date
2007
Authors
Desantis, S. ; Cirillo, F. ; Deflorio, M. ; Megalofonou, P. ; Palazón, J.L. ; Sarasquete, C. ; De Metrio, G.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The carbohydrate expression in the
epithelium lining the oesophagus of the toadfish
Halobatrachus didactylus was studied by means of
conventional and lectin histochemistry. The stratified
epithelium was constituted by basal cells, polymorphous
cells in the intermediate layer, pyramidal and flattened
cells in the outer layer and contained two types of large
secretory cells: goblet cells and sacciform cells. PAS,
Alcian blue pH 2.5 and pH 1.0 stained very strongly the
goblet cells, weakly the surface of the other epithelial
cells but did not stain the sacciform cells. The goblet
cells cytoplasm contained oligosaccharides with terminal
Galß1,3GalNAc, a/ßGalNAc, Galß1,4GlcNAc, aL-Fuc
and internal ßGlcNAc residues (PNA, SBA, RCA120,
UEA I, LTA and KOH-sialidase-WGA affinity).
Galß1,4GlcNAc, aL-Fuc and internal ßGlcNAc were
also found in the glycocalyx. The sacciform cells
expressed sialyloligosaccharides terminating with
Neu5Aca2,3Galß1,4GlcNac, Neu5Acß2,6Gal/GalNAc,
Neu5AcForssman pentasaccharide (MAL II, SNA,
KOH-sialidase-DBA staining) as well as asialoglycoconjugates
with terminal/internal aMan (Con A
affinity) and with terminal Galß1,3GalNAc, Forssman
pentasaccharide, Galß1,4GlcNAc, GalNAc (HPA and
SBA reactivity), aGal (GSA I-B4 reactivity), D-GlcNAc
(GSA II labelling), aL-Fuc. The basal cells cytoplasm
exhibited terminal/internal aMan and terminal
Neu5Aca2,6Gal/GalNAc, Galß1,4GlcNAc, a/ßGalNAc,
aGal, GlcNAc, aL-Fuc. Intermediate cells showed
oligosaccharides with terminal/internal aMan and/or
terminating with Neu5Aca2,6Gal/GalNAc,
Galß1,4GlcNAc in the cytoplasm and with
Neu5Aca2,3Galß1,4GlcNac, a/ßGalNAc, aGal,
GlcNAc, aL-Fuc in the glycocalyx. The pyramidal cells
expressed terminal/internal aMan and terminal
Neu5Aca2,6Gal/GalNAc, a/ßGalß1,4NAc, aGal, aLFuc in the entire cytoplasm, terminal Neu5Aca2,3
Galß1,4GlcNac and Forssman pentasaccharide in the
apical extension, internal ßGlcNAc and/or terminal aLFuc
in the luminal surface, Neu5aca2,3Galß1,4GlcNac,
Neu5Aca2,6Gal/GalNAc, Galß1,4GlcNAc, aGal in the
basolateral surface. The flattened cells displayed glycans
with terminal/internal aMan and terminal
Neu5Aca2,6Gal/GalNAc, a/ßGalNAc, aGal, DGlcNAc
in the entire cytoplasm, glycans terminating
with Galß1,3GalNAc and/or internal ßGlcNAc in the
sub-nuclear cytoplasm.
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