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The distribution and time-dependent expression of MAGL during skeletal muscle wound healing in rats

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dc.contributor.authorJiang, Shu-Kun
dc.contributor.authorZhang, Miao
dc.contributor.authorTian, Zhi-Ling
dc.contributor.authorWang, Lin-Lin
dc.contributor.authorZhao, Rui
dc.contributor.authorLi, Shan-shan
dc.contributor.authorLiu, Min
dc.contributor.authorWang, Meng
dc.contributor.authorGuan, Da-Wei
dc.date.accessioned2020-12-11T10:34:57Z
dc.date.available2020-12-11T10:34:57Z
dc.date.issued2015
dc.description.abstractMonoacylglycerol lipase (MAGL) is widely distributed in mammals and largely responsible for metabolizing 2-arachidonoylglycerol (2-AG). Little is known about its expression in skeletal muscles after trauma. A preliminary study on time-dependent expression and distribution of MAGL was performed by immunohistochemical staining, Western blotting and quantitative real-time PCR (qPCR) during skeletal muscle wound healing in rats. An animal model of skeletal muscle contusion was established in 40 Sprague-Dawley male rats. Samples were taken at 1, 3, 5, 7, 9, 13, 17 and 21 days after contusion, respectively (5 rats in each posttraumatic interval). 5 rats were employed as control. Weak immunoreactivity of MAGL was observed in the sarcoplasm of myofibers in control rats. Intensive immunoreactivties of MAGL were observed in polymorphonuclear cells (PMNs), roundshaped mononuclear cells (MNCs), spindle-shaped fibroblastic cells (FBCs) and regenerated multinucleated myotubes in the injured tissue. Subsequently, neutrophils, macrophages and myofibroblasts were identified as MAGL-positive cells by double immunofluorescent procedure. MAGL expression was remarkably up-regulated after contusion by qPCR and Western blot analysis. The results demonstrate that the expression of MAGL is distributed in certain cell types and time-dependently expressed in skeletal muscles after trauma, suggesting that MAGL may be involved in inflammatory response, fibrogenesis and muscle regeneration during skeletal muscle wound healing.es
dc.formatapplication/pdfes
dc.format.extent12es
dc.identifier.citationHistology and histopathology, Vol. 30, nº10, (2015)
dc.identifier.doi10.14670/HH-11-625
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/99807
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectSkeletal musclees
dc.subjectTraumaes
dc.subjectMAGLes
dc.subjectWound repaires
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleThe distribution and time-dependent expression of MAGL during skeletal muscle wound healing in ratses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30,nº10 (2015)