Publication: Effect of heparin and antivenom on skeletal muscle damage produced by Bothrops jararacussu venom
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Date
2002
Authors
Calil-Elias, S. ; Martinez, A.M.B. ; Melo, P.A.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
We examined the effect of treatment with
heparin and polyvalent antivenom on mice muscle
Extensor digitorum longus (EDL) regeneration, after
damage induced by injection of B o t h rops jara ra c u s s u
crude venom over the muscle of the right posterior limb.
The mice were separated into groups and each group
received treatment, by intravenous route with either high
molecular weight heparin (H), low molecular weight
heparin (LMWH), polyvalent antivenom (PAV) or with
the combination of PAV plus H or PAV plus LMWH at
15 minutes and 4 hours after the injection of the venom.
Myotoxicity was measured by the increase in plasma
creatine kinase (CK) activity at two hours after the
injection of the venom. The histological changes in EDL
at 1, 3, 7 and 21 days after the injection of the ve n o m
were analyzed by light microscopy. In each group the
normal and regenerated muscle fibers were quantifi e d
using Scion Image computer program. We also evaluated
in vitro, the influence of these substances in the
proteolytic and phospholipase activities of the ve n o m .
Heparins decreased the proteolytic activity of the venom
but did not affect its phospholipase activity. However the
PAV antagonized both activities. PAV and its
combinations showed antimyotoxic activity, according to
the magnitude of CK plasma levels. At 21 days the
r egeneration was observed in all animals, also in those that received only the venom. All treatments, ex c e p t
LMWH, promote a significant increase in the number of
muscle fibers.
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