Publication: The activity and proliferation of pituitary prolactin-positive cells and pituitary VIP-positive cells are regulated by interleukin 6
Authors
Blanco, Enrique J. ; Carretero-Hernández, Marta ; García-Barrado, Josefa ; Iglesias-Osma, Mª. Carmen ; Carretero, Manuel ; Herrero, Julio J. ; Rubio, Manuel ; Riesco, José M. ; Carretero, José
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Interleukins are proteins involved in the
immune system and have been related to the endocrine
regulation of the hypothalamic-pituitary-adrenal axis as
well as to the secretion of ACTH, prolactin (PRL), GH
and, possibly, LH. Like interleukin-6 (IL-6), vasoactive
intestinal peptide (VIP) is synthesized in the pituitary
gland and stimulates prolactin secretion. The aim of the
present study was to address whether Interleukin 6 is
involved in the regulation of VIP, as well as other factors
involved in the regulation of prolactin such as dopamine,
TRH and estradiol. Accordingly, we performed an in
vitro study on monolayer cultures of rat pituitary cells,
neutralizing the possible paracrine effect of IL-6 by
immunosuppressing the protein by treatment with
polyclonal antibody against IL-6 over 1, 3, 6 or 24 hours
and then determining the degree of proliferation of VIP
cells using double immunocytochemical labelling for
VIP or PRL and proliferating cell nuclear antigen
(PCNA). As a control, the effects of immunosuppression
on the proliferation of PRL-positive cells were analyzed.
Immunosuppression of IL-6 induced modifications in the
cellular and nuclear size of VIP-positive cells, indicating
an inhibitory process. Moreover, immunosuppression
induced a significant decrease in the proliferation rate of
PRL-positive or VIP-positive cells for all time-points
analyzed. Similar effects on the proliferation rate of
PRL-positive cells were found. The results of the present study demonstrate that IL-6 is involved in the regulation
of the activity and proliferation of pituitary VIPproducing
cells and suggest that, without ruling out a
direct effect of IL-6 on prolactin cells, IL-6 could
regulate prolactin by acting on pituitary VIP.
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Citation
Histology and Histopathology, vol. 28, nº 12 (2013)
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