Publication: Human resident CD34+ stromal
cells/telocytes have progenitor capacity
and are a source of αSMA+ cells during repair
Authors
Díaz-Flores, L. ; Gutiérrez, R. ; García, M.P. ; González, M. ; Sáez, F.J. ; Aparicio, F. ; Díaz-Flores Jr., L. ; Madrid Cuevas, Juan Francisco
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
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DOI
https://doi.org/10.14670/HH-30.615
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info:eu-repo/semantics/article
Description
Abstract
We studied the progenitor capacity of human
resident CD34+ stromal cells/telocytes (SC/TCs) in the
enteric wall affected by inflammatory/repair processes
(appendicitis, diverticulitis of large bowel and Crohn’s
disease of the terminal ileum) at different stages of
evolution (inflammatory, proliferative and remodelling).
In these conditions, CD34+ SC/TCs are activated,
showing changes, which include the following
overlapping events: 1) separation from adjacent
structures (e.g., from vascular walls) and location in
oedematous spaces, 2) morphological modifications (in
cell shape and size) with presence of transitional cell
forms between quiescent and activated CD34+ SC/TCs,
3) rapid proliferation and 4) loss of CD34 expression
and gain of αSMA expression. These events mainly
occur in the inflammatory and proliferative stages.
During the loss of CD34 expression, the following
findings are observed: a) irregular cell labelling intensity
for anti-CD34, b) co-localization of CD34 and actin, c)
concurrent irregular labelling intensity for αSMA and d)
αSMA expression in all stromal cells, with total loss of
CD34 expression. While CD34 expression was
conserved, a high proliferative capacity (Ki-67
expression) was observed and vice versa. In the
segments of the ileum affected by Crohn’s disease, the
stromal cells around fissures were αSMA+ and, in the
transitional zones with normal enteric wall, activated
CD34+ SC/TCs were observed. In conclusion, human
resident CD34+ SC/TCs in the enteric wall have
progenitor capacity and are activated with or without
differentiation into αSMA+ stromal cells during
inflammatory/repair processes.
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Citation
Histology and Histopathology, Vol. 30, n.º 5 (2015)
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