Publication: LncRNA HOTTIP mediated DKK1 downregulation confers metastasis and invasion in colorectal cancer cells
Authors
Rui, Yiqi ; Hu, Mingchao ; Wang, Peng ; Zhang, Chuanqiang ; Xu, Hua ; Li, Yuanzhong ; Zhang, Yu ; Gu, Jianchun ; Wang, Qiang
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Publisher
Universidad de Murcia. Departamento de BiologĂa Celular e HistologĂa
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DOI
DOI: 10.14670/HH-18-043
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info:eu-repo/semantics/article
Description
Abstract
Recent studies highlight long non-coding
RNAs (lncRNAs) as key regulators of cancer biology
that contribute to carcinogenesis. The lncRNA HOXA
transcript at the distal tip (HOTTIP) is involved in the
development of several cancers. Previous studies
demonstrated that HOTTIP could promote colorectal
cancer (CRC) cell proliferation via silencing of p21
expression. However, the potential role of HOTTIP in
CRC metastasis has not yet been discussed. Here, we
found that HOTTIP level was significantly higher in
CRC than in corresponding adjacent normal tissues, and
patients with a larger tumor size, advanced pathological
stage, or distant metastasis had higher HOTTIP
expression. Moreover, silencing HOTTIP expression by
siRNA or shRNA could inhibit CRC cell migration and
invasion in vitro and in vivo, whereas HOTTIP
overexpression promoted cell metastasis, as documented
in the SW480 cell lines. Mechanistic analyses indicated
that HOTTIP regulates CRC cell metastasis partly
through the downregulation of tumor suppressor DKK1
expression. Collectively, our results suggest that tumor
expression of lncRNA HOTTIP plays an important role
in CRC metastasis. HOTTIP may serve as a candidate
biomarker in this disease.
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Citation
Histology and Histopathology, Vol.34, nÂş6, (2019)
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Este Ătem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/