Browsing by Subject "LncRNA"

Now showing 1 - 4 of 4
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    LncRNA HOTTIP mediated DKK1 downregulation confers metastasis and invasion in colorectal cancer cells
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Rui, Yiqi; Hu, Mingchao; Wang, Peng; Zhang, Chuanqiang; Xu, Hua; Li, Yuanzhong; Zhang, Yu; Gu, Jianchun; Wang, Qiang
    Recent studies highlight long non-coding RNAs (lncRNAs) as key regulators of cancer biology that contribute to carcinogenesis. The lncRNA HOXA transcript at the distal tip (HOTTIP) is involved in the development of several cancers. Previous studies demonstrated that HOTTIP could promote colorectal cancer (CRC) cell proliferation via silencing of p21 expression. However, the potential role of HOTTIP in CRC metastasis has not yet been discussed. Here, we found that HOTTIP level was significantly higher in CRC than in corresponding adjacent normal tissues, and patients with a larger tumor size, advanced pathological stage, or distant metastasis had higher HOTTIP expression. Moreover, silencing HOTTIP expression by siRNA or shRNA could inhibit CRC cell migration and invasion in vitro and in vivo, whereas HOTTIP overexpression promoted cell metastasis, as documented in the SW480 cell lines. Mechanistic analyses indicated that HOTTIP regulates CRC cell metastasis partly through the downregulation of tumor suppressor DKK1 expression. Collectively, our results suggest that tumor expression of lncRNA HOTTIP plays an important role in CRC metastasis. HOTTIP may serve as a candidate biomarker in this disease.
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    LncRNA TUG1 aggravates cardiomyocyte apoptosis and myocardial ischemia/reperfusion injury
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2021) Fu, Dongliang; Gao, Tong; Liu, Mengru; Li, Chunyan; Li, Haiwei; Jiang, Hong; Li, Xianlun
    Cardiomyocyte apoptosis is a fundamental pathogenic factor leading to myocardial ischemia/ reperfusion (MI/R) injury. The long non-coding RNA (lncRNA) TUG1 regulates apoptosis in various cell types. We report here that TUG1 expression is induced in mouse heart following MI/R injury as well as in cardiomyocytes subjected to simulated ischemia/ reperfusion (SI/R) in vitro. Clinically, TUG1 expression is also elevated in plasma from patients with acute myocardial infarction (AMI), which implies its potential application as a disease biomarker. Functionally, TUG1 overexpression promotes, and its knockdown reduces SI/R-induced lactate dehydrogenase (LDH) release and caspase-3 activity in cardiomyocytes in vitro, illustrating that TUG1 exacerbates SI/R-induced apoptosis. Furthermore, in vivo, TUG1 aggravates MI/R injury in a mouse model, and subsequent observations show concurrent increased apoptosis of cardiomyocytes. Hence, this study unveils a clinical relevance and functional role of TUG1 in MI/R injury, and also implicates that targeting TUG1 m
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    Overexpression of lncRNA LINC01793 acts as a potential predictor for progression and poor prognosis of gastric cancer
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2019) Huang, Shujun; Chen, Lihua
    Objective. Accumulating evidence has shown that differentially expressed long non-coding RNA (lncRNA) is closely related to the development of gastric cancer. This study aims to explore the role of potential lncRNAs in the development of gastric cancer. Methods. TCGA database of gastric cancer were analyzed by bioinformatics. LINC01793 was overexpressed in gastric cancer. Furthermore, LINC01793 level in 21 pairs of gastric cancer and paracancerous tissues were detected by qRT-PCR (quantitative real-time polymerase chain reaction). Prognostic analysis was performed to investigate the predictive effect of LINC01793 on the prognosis of patients with gastric cancer. GSEA analysis was performed to investigate the possible biological processes of LINC01793 involved in the development of gastric cancer. Results. LINC01793 was overexpressed in gastric cancer tissues compared to paracancerous tissues. Moreover, LINC01793 overexpression in gastric cancer was closely related to patients with poor prognosis. GSEA analysis found that LINC01793 was closely related to apoptosis, cell cycle, invasion and metastasis of gastric cancer cells. Conclusions. Our study showed that overexpression of LINC01793 indicates the poor prognosis of gastric cancer patients. Therefore, LINC01793 may serve as a target for the diagnosis and treatment of gastric cancer.
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    The regulatory roles and potential prognosis implications of long non-coding RNAs in gastric cancer
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Wang, Yue; Yang, Fan; Yang, Qing
    Accumulating dysregulated lncRNAs have been demonstrated to execute vital functions in the pathogenesis and progress of gastric cancer (GC) through versatile molecular mechanisms. In this review, we classify the mechanisms of dysregulated lncRNAs in GC into several governing types according to their roles at molecular level. For each regulatory role, we illustrate several instructive examples and introduce significant effects of lncRNAs on cellular biological properties of GC. Besides, we summarize a group of lncRNA- signatures that are potential biomarkers in the prediction of prognosis for GC patients.