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Cellular proliferation, differentiation and apoptosis in polyether-polyurethane sponge implant model in mice

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dc.contributor.authorCampos, Paula P.es
dc.contributor.authorAndrade, Silvia P.es
dc.contributor.authorMoro, L.
dc.contributor.authorFerreira, M.A.N.D.
dc.contributor.authorVasconcelos, A.C.
dc.date.accessioned2011-06-30T12:02:06Z
dc.date.available2011-06-30T12:02:06Z
dc.date.issued2006
dc.description.abstractThe integration of implanted material to host organism requires spatial and temporal organization of several cellular processes, such as proliferation, differentiation and apoptosis. Despite the clinical relevance of these processes, there is little information regarding the sequence of such events in synthetic matrices. Here, we present a combination of techniques used to characterize the fibrovascular response in subcutaneous polyether-polyurethane sponge implants in mice at days 4, 7, 10 and 14 postimplantation. The AgNOR technique was modified and used as a surrogate marker for proliferating and activated cells invading the implant. The number of AgNOR-stained cells increased progressively from day 4 (606±76) to day 14 (2146±71) postimplantation. The number of TUNEL-positive (apoptotic index) cells also increased progressively from day 4 (459±40) to day 14 (1157±119) postimplantation. However, the ratio of TUNEL-labeled/proliferating cells had its highest peak in the early phase of the process remaining stable until day 14. Using Picrosirius staining it was shown that thin collagen increased from day 4, peaking at day 10 and falling markedly at day 14, whereas dense collagen increased progressively during the whole period. These experiments hold potential to investigate not only distinct phases of tissue repair induced by synthetic matrices but also to study underlying mechanisms involvedes
dc.formatapplication/pdfes
dc.format.extent8es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/22611
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectSponge implantses
dc.subjectCollagenes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleCellular proliferation, differentiation and apoptosis in polyether-polyurethane sponge implant model in micees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.21, nº12 (2006)