Publication: Cellular proliferation, differentiation and apoptosis in polyether-polyurethane sponge implant model in mice
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Date
2006
Authors
Campos, Paula P. ; Andrade, Silvia P. ; Moro, L. ; Ferreira, M.A.N.D. ; Vasconcelos, A.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The integration of implanted material to host
organism requires spatial and temporal organization of
several cellular processes, such as proliferation,
differentiation and apoptosis. Despite the clinical
relevance of these processes, there is little information
regarding the sequence of such events in synthetic
matrices. Here, we present a combination of techniques
used to characterize the fibrovascular response in
subcutaneous polyether-polyurethane sponge implants in
mice at days 4, 7, 10 and 14 postimplantation. The
AgNOR technique was modified and used as a surrogate
marker for proliferating and activated cells invading the
implant. The number of AgNOR-stained cells increased
progressively from day 4 (606±76) to day 14 (2146±71)
postimplantation. The number of TUNEL-positive
(apoptotic index) cells also increased progressively from
day 4 (459±40) to day 14 (1157±119) postimplantation.
However, the ratio of TUNEL-labeled/proliferating cells
had its highest peak in the early phase of the process
remaining stable until day 14. Using Picrosirius staining
it was shown that thin collagen increased from day 4,
peaking at day 10 and falling markedly at day 14,
whereas dense collagen increased progressively during
the whole period. These experiments hold potential to
investigate not only distinct phases of tissue repair
induced by synthetic matrices but also to study
underlying mechanisms involved
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