Publication:
Effects of single, double or triple combinations of octreotide, galanin and serotonin on a human pancreatic cancer cell line

Simple item page
dc.contributor.authorTjomsland, V.es
dc.contributor.authorEl-Salhy, M.es
dc.date.accessioned2011-06-30T12:00:23Z
dc.date.available2011-06-30T12:00:23Z
dc.date.issued2005
dc.description.abstractThe human pancreatic cancer cell line (SW 1990) was exposed to 0.2 µg/ml of octreotide, galanin or serotonin as single, double or triple combinations. The tumor cells were checked at 3, 6 and 12 hours. In order to determine the number of viable cancer cells, the MTTassay was used. Proliferation, apoptosis and the expression of epidermal growth factor were detected with immunohistochemistry using the avidin-biotin complex method. In addition, apoptosis was also detected with (TUNEL) method. The primary antibodies used were proliferating cell nuclear antigen (PCNA), anti-poly (ADP-ribose) polymerase (PARP) and antihuman epidermal growth factor. Single treatment with octreotide or serotonin reduced, the number of viable cells and the proliferation index at all observation times. Galanin increased the number of viable cells and the proliferation index. Whereas double treatments containing octreotide reduced the number of viable cells, those containing galanin increased the number. The effect of single, double or triple treatment on the apoptotic index obtained with both TUNEL method and PARP expression varied depending on the combination and the observation time. Octreotide did not affect the tumor cell expression of EGF. Galanin and serotonin, on the other hand, increased the expression of EGF Whereas triple combination increased the expression of EGF after 6 h, all the other double combinations decreased this expression. It has been concluded that treatment with a combination of octreotide and serotonin may be useful in clinical settings.es
dc.formatapplication/pdfes
dc.format.extent5es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/22487
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectPancreatic canceres
dc.subjectSerotonines
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleEffects of single, double or triple combinations of octreotide, galanin and serotonin on a human pancreatic cancer cell linees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Effects of single double or triple.pdf
Size:
1.23 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.20, nº 2 (2005)