Publication: Effects of single, double or triple combinations of octreotide, galanin and
serotonin on a human pancreatic cancer cell line
Authors
Tjomsland, V. ; El-Salhy, M.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The human pancreatic cancer cell line (SW
1990) was exposed to 0.2 µg/ml of octreotide, galanin or
serotonin as single, double or triple combinations. The
tumor cells were checked at 3, 6 and 12 hours. In order
to determine the number of viable cancer cells, the MTTassay
was used. Proliferation, apoptosis and the
expression of epidermal growth factor were detected
with immunohistochemistry using the avidin-biotin
complex method. In addition, apoptosis was also
detected with (TUNEL) method. The primary antibodies
used were proliferating cell nuclear antigen (PCNA),
anti-poly (ADP-ribose) polymerase (PARP) and antihuman
epidermal growth factor. Single treatment with
octreotide or serotonin reduced, the number of viable
cells and the proliferation index at all observation times.
Galanin increased the number of viable cells and the
proliferation index. Whereas double treatments
containing octreotide reduced the number of viable cells,
those containing galanin increased the number. The
effect of single, double or triple treatment on the
apoptotic index obtained with both TUNEL method and
PARP expression varied depending on the combination
and the observation time. Octreotide did not affect the
tumor cell expression of EGF. Galanin and serotonin, on
the other hand, increased the expression of EGF Whereas triple combination increased the expression of
EGF after 6 h, all the other double combinations
decreased this expression. It has been concluded that
treatment with a combination of octreotide and serotonin
may be useful in clinical settings.
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