Publication: Tripartite motif-containing 35 (TRIM35) is up-regulated in UUO-induced renal fibrosis animal model
Authors
Chen, Yu ; Ding, Yue ; Wang, Li-Ming
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-255
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info:eu-repo/semantics/article
Description
Abstract
Renal fibrosis has been recognized as a
serious health threat in the world because of the high
cost of treatment and poor prognosis. However, the
molecular mechanism of renal fibrosis is still largely
unknown. In this study, we aimed at illustrating the role
of TRIM35 in the renal fibrosis process. A UUO mouse
model and a TGF-β1-induced tubulointerstitial fibrosis
model were constructed for the research of renal fibrosis
at animal and cell level, respectively. Hematoxylin-eosin
and Masson staining were used for visualizing the
pathological change. qRT-PCR, Western blot analysis
and immunohistochemical staining were used to detect
the expression of fibrosis-associated proteins and
TRIM35. The results showed that, after the modeling,
the expressions of α-SMA, Collagen I, Collagen III,
Fibronectin and Snail1 were up-regulated, while the
expression of E-cadherin was down-regulated, indicating
the successful construction of animal and cell models.
More importantly, TRIM35 was proved to be upregulated in both animal and cell models. Therefore, this
study demonstrates the potential promotional effect of
TRIM35 in the renal fibrosis process, which may prove
to be a new biomarker for the diagnosis and
development of new treatments of renal fibrosis.
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Citation
Histology and Histopathology Vol. 35, nº12 (2020)
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