Publication: Long non-coding RNA XIST promotes the malignant features of oral squamous cell carcinoma (OSCC) cells through regulating miR-133a-5p/VEGFB
Authors
Wu, Kankui ; Wu, Mengxuan ; Wu, Wancui ; Liu, Wenzhe
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-504
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info:eu-repo/semantics/article
Description
Abstract
Objective. Oral squamous cell carcinoma
(OSCC) represents a frequently seen oral cavity
malignancy, and the mechanisms of its occurrence and
development remain unclear. The present work
examined the expression and biological function of long
non-coding RNA (lncNRA) XIST (X-inactive specific
transcript) in OSCC cells and tissues.
Study design. A total number of 50 OSCC and
paired non-carcinoma tissue samples were collected in
this study. Gene expression levels in cancer tissues and
cells were quantified by RT-qPCR. In addition, gain- and
loss-of-function experiments were conducted to
investigate the biological roles of XIST as well as its
downstream targets in OSCC cells.
Results. XIST was upregulated in OSCC cells and
tissues, which predicted a poorer prognostic outcome in
OSCC patients. Silencing XIST inhibited the growth and
invasion of OSCC cells and triggered apoptosis. miR133a-5p was identified as a downstream target of XIST,
which was downregulated in OSCC tissues. miR-133a5p mediated the effect of XIST by targeting VEGFB.
VEGFB overexpression rescued the inhibitory effects of
XIST silencing on cell growth, invasion and migration.
Conclusion. Taken together, the above data indicates
that XIST serves as an oncogenic factor to enhance the
growth and invasion of
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Citation
Histology and Histopathology Vol. 38, nº1 (2023)
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