Publication:
Regulation of hepatocyte glutathione content by hepatic sinusoidal cells activated with LPS: anatomical restrictions

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dc.contributor.authorCatalá, Myrianes
dc.contributor.authorPagani, Raffaellaes
dc.contributor.authorPortolés, M.Teresa
dc.date.accessioned2014-01-09T10:39:06Z
dc.date.available2014-01-09T10:39:06Z
dc.date.issued2009
dc.description.abstractThe liver is the main organ for the elimination of bacterial endotoxin involving Kupffer and parenchymal cells. This process is accompanied by the release of free radicals. Parenchymal cells possess especially high levels of glutathione, which make them a key point in the response to free radicals. Sinusoidal cells regulate hepatic function in a very important fashion through the release of cytokines and/or adhesion molecules. These facts suggest the importance of finding new in vitro experimental models representing an intermediate step towards in vivo models. The treatment with LPS of sinusoidal and parenchymal cell co-cultures on porous membranes provokes an intense reduction of parenchymal cell intracellular glutathione, which does not correspond to in vivo results. However, the addition of supernatants of LPS-treated sinusoidal cells to parenchymal cells renders increases in glutathione which agree better with in vivo results. We conclude that the regulation of liver hepatocyte glutathione content and NO release in the presence of LPS is strongly modulated by liver non parenchymal cells. The study of this phenomenon requires new in vitro models taking into account liver histophysiology and histopathology and anatomical restrictions in cell communication.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/37558
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHepatocytees
dc.subjectKupffer cellses
dc.subject.other577 - Bioquímica. Biología molecular. Biofísicaes
dc.titleRegulation of hepatocyte glutathione content by hepatic sinusoidal cells activated with LPS: anatomical restrictionses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.24,nº12 (2009)