Publication: Regulation of hepatocyte glutathione content by hepatic sinusoidal cells activated with LPS: anatomical restrictions
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Date
2009
Authors
Catalá, Myrian ; Pagani, Raffaella ; Portolés, M.Teresa
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The liver is the main organ for the
elimination of bacterial endotoxin involving Kupffer and
parenchymal cells. This process is accompanied by the
release of free radicals. Parenchymal cells possess
especially high levels of glutathione, which make them a
key point in the response to free radicals. Sinusoidal
cells regulate hepatic function in a very important
fashion through the release of cytokines and/or adhesion
molecules. These facts suggest the importance of finding
new in vitro experimental models representing an
intermediate step towards in vivo models. The treatment
with LPS of sinusoidal and parenchymal cell co-cultures
on porous membranes provokes an intense reduction of
parenchymal cell intracellular glutathione, which does
not correspond to in vivo results. However, the addition
of supernatants of LPS-treated sinusoidal cells to
parenchymal cells renders increases in glutathione which
agree better with in vivo results. We conclude that the
regulation of liver hepatocyte glutathione content and
NO release in the presence of LPS is strongly modulated
by liver non parenchymal cells. The study of this
phenomenon requires new in vitro models taking into
account liver histophysiology and histopathology and
anatomical restrictions in cell communication.
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