Publication: Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro
| dc.contributor.author | Weischer, C.H. | es |
| dc.contributor.author | Krisinger, J. | es |
| dc.contributor.author | Karzel, K. | |
| dc.date.accessioned | 2011-01-17T12:39:22Z | |
| dc.date.available | 2011-01-17T12:39:22Z | |
| dc.date.issued | 1986 | |
| dc.description.abstract | The aim of this studv was to develop a standardized image analysis method for localization and quantitative measurement of calcified structures of murine fetal tibiae cultured in vitro as a completion and verification of previous biochemical studies. The calcified structures of bone stained by von Kossa silver technique and the epiphyseal cartilages showing intensive metachromasia with toluidine-blue staining were converted with grey-value window programs and afterwards the areas of the selected structures were measured. The histomorphological investigations showed that the murine tibiae, incubated for a period of 6 days in a medium with addition of 5 mmol mellitic acid, showed both a significant reduction of calcium deposits and an increase of epiphyseal intercellular cartilage matrix. The tibiae incubated in a medium with addition of 0.5 mmol sodium fluoride significantly showed an increase of calcium deposits in the thickened lammellae of the cornpacta. These histomorphological results confirm previous biochemical studies. | en_EN |
| dc.format | application/pdf | es |
| dc.format.extent | 6 | es |
| dc.identifier.issn | 0213-3911 | es |
| dc.identifier.uri | http://hdl.handle.net/10201/17832 | |
| dc.language | eng | es |
| dc.publisher | Murcia : F. Hernández | es |
| dc.relation.ispartof | Histology and histopathology | es |
| dc.rights | info:eu-repo/semantics/openAccess | es |
| dc.subject | Mellitic acid (MA) | es |
| dc.subject | Sodium fluoride (NaF) | es |
| dc.subject.other | CDU::6 - Ciencias aplicadas::61 - Medicina | es |
| dc.title | Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro | es |
| dc.type | info:eu-repo/semantics/article | es |
| dspace.entity.type | Publication | es |
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