Publication: Effects of mellitic acid (MA) and sodium fluoride (NaF) on the histological appearance of murine fetal tibiae cultured in vitro
Loading...
Date
1986
Authors
Weischer, C.H. ; Krisinger, J. ; Karzel, K.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The aim of this studv was to develop a
standardized image analysis method for localization and
quantitative measurement of calcified structures of murine
fetal tibiae cultured in vitro as a completion and verification
of previous biochemical studies. The calcified structures of
bone stained by von Kossa silver technique and the
epiphyseal cartilages showing intensive metachromasia with
toluidine-blue staining were converted with grey-value
window programs and afterwards the areas of the selected
structures were measured. The histomorphological
investigations showed that the murine tibiae, incubated for
a period of 6 days in a medium with addition of 5 mmol
mellitic acid, showed both a significant reduction of calcium
deposits and an increase of epiphyseal intercellular cartilage
matrix. The tibiae incubated in a medium with addition of
0.5 mmol sodium fluoride significantly showed an increase
of calcium deposits in the thickened lammellae of the
cornpacta. These histomorphological results confirm
previous biochemical studies.
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.