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Light microscopic catalase histochemistry in mussel digestive gland tissue

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dc.contributor.authorCajaraville, M.P.es
dc.contributor.authorUranga, J.A.es
dc.contributor.authorAngulo, E.
dc.date.accessioned2011-02-01T09:16:46Z
dc.date.available2011-02-01T09:16:46Z
dc.date.issued1993
dc.description.abstractDifferent light microscopical procedures for the histochemical demonstration of catalase were tested in cryostat sections of mussel digestive gland tissue by using both benzidine and diaminobenzidine (DAB) as hydrogen donors. The selected procedure, which was also applied to mouse liver for comparative purposes, consisted of incubation in media containing 0.2% DAB and 0.3% H202 at pH 10.4 for 35 min at 42 "C. Addition of 0.01 M imidazole to the incubation medium increased the staining intensity of the histochemical procedure. The positive reaction product was localized in epithelial cells lining the digestive tubules and the ducts. The histochemical reaction was inhibited partially by aminotriazole or sodium azide and disappeared completely by omission of H202 from the incubation medium. On the other hand, heat resistant nonenzymatic reactions were observed in sites known to contain lipofuscins such as epithelial cells of the gastrointestinal tract and connective tissue brown cells.es
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18418
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCatalasees
dc.subjectHistochemistryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleLight microscopic catalase histochemistry in mussel digestive gland tissuees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol. 8, nº 3 (1993)