Publication: Quantitative immunohistochemical assessment of blood and lymphatic microcirculation in cutaneous lichen planus lesions
Authors
Výbohová, Desanka ; Mellová, Yvetta ; Adamicová, Katarína ; Adamkov, Marián ; Hešková, Gabriela
item.page.secondaryauthor
item.page.director
Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
10.14670/HH-30.697
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Latest advances have brought to light the
hypothesis that angiogenesis and lymphangiogenesis are
tightly connected to some chronic inflammatory
diseases. The present study focuses on immunohistochemical assessment of the quantitative changes in the
blood and lymphatic microcirculatory bed in common
chronic dermatosis - cutaneous lichen planus. Double
immunohistochemistry with CD34 and podoplanin
antibodies was used to detect blood and lymphatic
endothelium, while anti-human VEGF was used for the
observation of a key angiogenesis and lymphangiogenesis inducer. Morphometric analysis was performed
with QuickPhoto Micro image analysis software. Results
confirmed statistically significant enlargement of both
the blood and lymphatic microcirculatory beds.
Compared to healthy skin, cutaneous lichen planus
lesions revealed 1.6 times enlarged blood
microcirculatory bed and 1.8 times enlarged lymphatic
microcirculatory bed. Vascular endothelial growth factor
(VEGF) expression in lesional skin was significantly
higher in the epidermis (19.1 times increase) than in the
dermis (10.3 times increase). These findings indicate a
tight association of angiogenesis and lymphangiogenesis
with the pathogenesis of cutaneous lichen planus.
publication.page.subject
Citation
Histology and Histopathology, vol.30, nº 6, (2015)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/