Publication: An electron microscopic and biochemical study of the effects of propranolol on the
glycogen autophagy in newborn rat hepatocytes
Authors
Kotoulas, Othon B. ; Kalamidas, Stefanos ; Miles, P. ; Hann, A.C.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effects of propranolol on the glycogen
autophagy in newborn rat hepatocytes were studied by
using biochemical determinations, electron microscopy
and morphometric analysis. Propranolol lowered the
liver cyclic AMP and cyclic AMP-dependent protein
kinase activity. It also decreased the formyl-methionylleucyl-
phenylalanine (FMLP)-inhibitable Ca2+-ATPase
activity including lysosomal calcium uptake pump. The
normal postnatal increase in the volume of autophagic
vacuoles and the activity of acid glycogen-hydrolyzing
alpha glucosidase were inhibited. Also, the degradation
of glycogen inside the autophagic vacuoles was
apparently inhibited. The activity of acid mannose 6-
phosphatase was increased. These findings indicate that
propranolol influences several steps in the sequence of
events leading to the breakdown of glycogen in the
autophagic vacuoles of newborn rat hepatocytes. This
supports our previous studies suggesting that cyclic
AMP regulates glycogen autophagy.
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Citation
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