Browsing by Subject "Glycogen"

Now showing 1 - 4 of 4
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    An electron microscopic and biochemical study of the effects of propranolol on the glycogen autophagy in newborn rat hepatocytes
    (Murcia : F. Hernández, 2003) Kotoulas, Othon B.; Kalamidas, Stefanos; Miles, P.; Hann, A.C.
    The effects of propranolol on the glycogen autophagy in newborn rat hepatocytes were studied by using biochemical determinations, electron microscopy and morphometric analysis. Propranolol lowered the liver cyclic AMP and cyclic AMP-dependent protein kinase activity. It also decreased the formyl-methionylleucyl- phenylalanine (FMLP)-inhibitable Ca2+-ATPase activity including lysosomal calcium uptake pump. The normal postnatal increase in the volume of autophagic vacuoles and the activity of acid glycogen-hydrolyzing alpha glucosidase were inhibited. Also, the degradation of glycogen inside the autophagic vacuoles was apparently inhibited. The activity of acid mannose 6- phosphatase was increased. These findings indicate that propranolol influences several steps in the sequence of events leading to the breakdown of glycogen in the autophagic vacuoles of newborn rat hepatocytes. This supports our previous studies suggesting that cyclic AMP regulates glycogen autophagy.
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    Histological changes of liver glycogen storage in mice (Mus musculus) caused by high-protein diets
    (Murcia : F. Hernández, 2006) Ulusoy, E.; Eren, B.
    High Protein diets (HP) have been popular for people who want to lose weight since the 1960s. Even though these diets do not harm healthy people in the short term, there is insufficient data to support their safe use and efficiency over a long period. Because of the fact that the proteins in these diets are mainly from animal sources, it induces a higher intake of total fat, saturated fat and cholesterol. It is proven that high protein diets cause both physical and pathological abnormalities in the body. However, there exist very few studies about the effects of high protein nutrition on liver glycogen storage. For this study 40 Swiss albino mice consisting of two groups were used. The first group was fed with 25% High Protein; the other was fed with standard meal. The two groups were fed with respect to their diets for 30 days. At the end of 15th, 20th, 25th and 30th days 5 from each group were killed with cervical dislocation. The livers were removed after perfusion then fixated. The routine paraffin pursuit was applied before cutting into 5 µm sections and staining with H-E, PAS and silver. There were major differences in weight loss between the first and the fifteenth days. Glycogen storage was significantly reduced in HP (15) stained with PAS. Hydropic degeneration and regenerative activity was observed in H-E and silver stained HP group. As a result for the high protein diet group, weight loss at the 15th day and a significant decrement in glycogen storage at the 30th day was observed.
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    Reposición del Glucógeno Muscular en la Recuperación del Deportista
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2019) Mata-Ordoñez, Fernando; Grimaldi-Puyana, Moisés; Sánchez-Oliver, Antonio Jesús
    Las estrategias nutricionales durante la fase de recuperación del deportista son fundamentales. Uno de los principales objetivos de la recu- peración es la reposición del glucógeno muscular. Este aspecto se hace más importante cuando los deportistas se enfrentan a entrenamientos intensos o eventos competitivos con cortos periodos de recuperación. Además, la manipulación deliberada de su disponibilidad puede mejorar las adaptacio- nes moleculares al entrenamiento. La presente revisión tiene por objetivo informar sobre los aspectos fisiológicos básicos de esta situación, así como conocer el momento del consumo, la cantidad, el tipo y la interacción de diferentes nutrientes con los hidratos de carbono, para poder maximizar o jugar con la reposición del mismo en función de las necesidades y/o las estrategias planteadas. El glucógeno ya no debe ser visto como un simple almacén de energía sino como una molécula que puede desencadenar nu- merosos procesos celulares importantes para el deportista.
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    The breakdown of glycogen in the lysosomes of newborn rat hepatocytes: The effects of glucose, cyclic 3',5'-AMP and caffeine
    (Murcia : F. Hernández, 1994) Kalamidas, Stefanos; Kotoulas, Othon B.; Kotoulas, Angeliki O.; Maintas, D.B.
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