Publication:
Isolation of functional mature peritoneal macrophages from healthy humans.

Simple item page
dc.contributor.authorMartínez Banaclocha, Helios
dc.contributor.authorMarín Sánchez, Pilar
dc.contributor.authorCarmona Martínez, Violeta
dc.contributor.authorIniesta Albadalejo, Miguel Ángel
dc.contributor.authorTristán Manzano, María
dc.contributor.authorGarcía Peñarrubia, Pilar
dc.contributor.authorMachado Linde, Francisco
dc.contributor.authorMartínez-Esparza Alvargonzález, María Concepción
dc.contributor.authorPelegrín Vivancos, Pablo
dc.contributor.authorRuiz Alcaraz, Antonio José
dc.contributor.authorTapia Abellán, Ana
dc.contributor.departmentBioquímica y Biología Molecular B e Inmunología
dc.date.accessioned2024-06-26T11:40:14Z
dc.date.available2024-06-26T11:40:14Z
dc.date.issued2019-11-06
dc.description© 2019 Australian and New Zealand Society for Immunology Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted version of a Published Work that appeared in final form in Immunology & Cell Biology. To access the final edited and published work see https://doi.org/10.1111/imcb.12305
dc.description.abstractMacrophages play an important role in the inflammatory response. Their various biological functions are induced by different membrane receptors, including Toll-like receptors, which trigger several intracellular signaling cascades and activate the inflammasomes, which in turn elicit the release of inflammatory mediators such as cytokines. In this study, we present a novel method for the isolation of human mature peritoneal macrophages. This method can be easily implemented by gynecologists who routinely perform laparoscopy for sterilization by tubal ligation or surgically intervene in benign gynecological pathologies. Our method confirms that macrophages are the main peritoneal leukocyte subpopulation isolated from the human peritoneum in homeostasis. We showed that primary human peritoneal macrophages present phagocytic and oxidative activities, and respond to activation of the main proinflammatory pathways such as Toll-like receptors and inflammasomes, resulting in the secretion of different proinflammatory cytokines. Therefore, this method provides a useful tool for characterizing primary human macrophages as control cells for studies of molecular inflammatory pathways in steady-state conditions and for comparing them with those obtained from pathologies involving the peritoneal cavity. Furthermore, it will facilitate advances in the screening of anti-inflammatory compounds in the human system.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.citationImmunology & Cell Biology, 2020, Vol. 98, N. 2, pp. 114-126
dc.identifier.doihttps://doi.org/10.1111/imcb.12305
dc.identifier.issnPrint: 0818-9641
dc.identifier.issnElectronic: 1440-1711
dc.identifier.urihttp://hdl.handle.net/10201/142711
dc.languageenges
dc.publisherWileyes
dc.relationThe study was supported by the Precipita crowdfunding platform (PR200; Endometriosis to PG-P), Fundación Española para la Ciencia y la Tecnología Ministerio de Ciencia y Universidades, Spain, the Ministerio de Economía, Industria y Competitividad (SAF2017-88276-R to PP), the Fundación Séneca (20859/PI/18 to PP) and the European Research Council (ERC-2013-CoG 614578 to PP). HM-B was supported by a Rio Hortega fellowship from the Instituto Salud Carlos III (CM14/00008). VC-M was supported by the Dirección General de Investigación Científica y Técnica and Fondo Social Europeo (EJ-2014-A-95803), Spain.es
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/imcb.12305es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCytokineses
dc.subjectHuman macrophageses
dc.subjectInflammationes
dc.subjectInnate immunityes
dc.subjectPeritoneal leukocyteses
dc.titleIsolation of functional mature peritoneal macrophages from healthy humans.es
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication79449fb4-f715-4255-a064-bfecc4b502de
relation.isAuthorOfPublication547c4c2c-d906-4e1d-ae9a-0cdb40eef6d1
relation.isAuthorOfPublicationc5d54ef3-bd3b-4330-b4a1-252ab278925b
relation.isAuthorOfPublication0ef289d2-ad85-4d81-a66c-82879daf26a9
relation.isAuthorOfPublication.latestForDiscovery79449fb4-f715-4255-a064-bfecc4b502de
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
16618530.pdf
Size:
1.45 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
2.26 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Artículos