Publication: Isolation of functional mature peritoneal macrophages from healthy humans.
Authors
Martínez Banaclocha, Helios ; Marín Sánchez, Pilar ; Carmona Martínez, Violeta ; Iniesta Albadalejo, Miguel Ángel ; Tristán Manzano, María ; García Peñarrubia, Pilar ; Machado Linde, Francisco ; Martínez-Esparza Alvargonzález, María Concepción ; Pelegrín Vivancos, Pablo ; Ruiz Alcaraz, Antonio José ; Tapia Abellán, Ana
item.page.secondaryauthor
item.page.director
Publisher
Wiley
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1111/imcb.12305
item.page.type
info:eu-repo/semantics/article
Description
© 2019 Australian and New Zealand Society for Immunology Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Immunology & Cell Biology. To access the final edited and published work see https://doi.org/10.1111/imcb.12305
Abstract
Macrophages play an important role in the inflammatory response. Their
various biological functions are induced by different membrane receptors,
including Toll-like receptors, which trigger several intracellular signaling
cascades and activate the inflammasomes, which in turn elicit the release of
inflammatory mediators such as cytokines. In this study, we present a novel
method for the isolation of human mature peritoneal macrophages. This
method can be easily implemented by gynecologists who routinely perform
laparoscopy for sterilization by tubal ligation or surgically intervene in benign
gynecological pathologies. Our method confirms that macrophages are the
main peritoneal leukocyte subpopulation isolated from the human peritoneum
in homeostasis. We showed that primary human peritoneal macrophages
present phagocytic and oxidative activities, and respond to activation of the
main proinflammatory pathways such as Toll-like receptors and
inflammasomes, resulting in the secretion of different proinflammatory
cytokines. Therefore, this method provides a useful tool for characterizing
primary human macrophages as control cells for studies of molecular
inflammatory pathways in steady-state conditions and for comparing them
with those obtained from pathologies involving the peritoneal cavity.
Furthermore, it will facilitate advances in the screening of anti-inflammatory
compounds in the human system.
publication.page.subject
Citation
Immunology & Cell Biology, 2020, Vol. 98, N. 2, pp. 114-126
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/